- Dave Foley as Flik
- Jerome Ranft as Heimlich
- Julia Louis-Dreyfus as Queen Atta
- Hayden Panettiere as Princess Dot
- Denis Leary as Francis
- David Hyde Pierce as Slim
- Jonathan Harris as Manny
- Macaulay Culkin as Kevin
- Madeline Kahn as Gypsy
- Bonnie Hunt as Rosie
- Sam Witwer as Lord Krypton
- Michael McShane as Tuck and Roll
- John Ratzenberger as P.T. Flea
- Brad Garrett as Dim
- Nev Scharrel as Boba main protagonist of A Bug's Life 2
- as Lord Xur
- Roddy McDowall as Mr. Soil
- Edie McClurg as Dr. Flora
- Alex Rocco as Thorny
- David Ossman as Cornelius